Image Stack Merger Plus is a plugin for ImageJ. As ImageJ, Image Stack Merger Plus is FREE and Open Source.

 

This ImageJ plugin permits from a video converted to image sequence to delete the moving subjects and create a clean background reference image. Then you will be able to sustract this reference image to all your sequence.

 Click to enlarge !

 See the commented demonstration

 Download plugin & sources !

 

 Download the archive file and extract it in your ImageJ plugin folder (usually C:Program FilesImageJplugins for Windows). Then restart ImageJ or use Help > Refresh Menus.